Workshop in Microscopy and Quantitative Histology

University of Bonn and the Bonn International Graduate Studies (BIGS) Program

The goal of this workshop is to combine formalized lectures on the principles of microscopy and quantitative histology (stereology) with practical sessions on the use of microscopes and post-acquisition analysis. Upon completing this short course, participants should be able to efficiently operate their research instruments, obtain optimal image resolution and quality, and understand the design of a quantitative stereological study.

The participants are expected to be primarily doctoral students and postdoctoral fellows. Registration is required (see below) but there is no cost to attend. Lunch will be provided. Enrollment in the lectures is open to all PhD students and post-doctoral fellows. Registration in the practical sessions is limited to 20 participants. A brief Statement of Interest will be used to select the Practicum participants, with preference given to graduate students.

2-4 December, 2015
Life and Brain Institute, Large Seminar Room
University of Bonn Medical Center, Germany



Instruction is by Prof. Dr. Daniel A. Peterson, Professor and Vice-Chairman of Neuroscience and Director, Center for Stem Cell and Regenerative Medicine, The Chicago Medical School. Prof Dr. Peterson has been a Fulbright Senior Scholar and Visiting Professor at the Institute for Reconstructive Neurobiology at UniKlinikum Bonn.




Program of Instruction (Flyer)

2 December, 2015      Principles of Widefield and Confocal Microscopy

09:00 – 12:00               Principles of Microscopy, Fluorescence, and Image Acquisition

13:00 – 17:00               Directed group tutorials at the confocal microscope

Enrollment limited to 20


3 December, 2015      Principles of Quantitative Histology and Systematic Sampling

09:00 – 12:00               Introduction to Design-Based Stereology and Estimating Cell Number

13:00 – 17:00               Estimation of Volume and Length; Sampling Design and Efficiency


4 December, 2015      Practicum on Photoshop and Quantitative Histology

Whole day, Enrollment limited to 20

09:00 – 12:00               Post-Processing of Images for Scientific Analysis and Publication

14:00 – 17:00               Designing and Implementing a Stereological Study

Because of a high request we will offer a further practical course on 5 December and 11 December, 2015.

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Promoted by the German Academic Exchange Service, and the Alexander von Humboldt-Foundation

and funded by the Federal Ministry of Education and Research