University of Bonn and the Bonn International Graduate School (BIGS) PhD Program
The goal of this workshop is to combine formalized lectures on the principles of microscopy and quantitative histology (stereology) with practical sessions on the use of confocal microscopes and post-acquisition analysis. Upon completing this short course, participants should be able to efficiently operate their research instruments, obtain optimal image resolution and quality, and understand the design of a quantitative stereological study.
The participants are expected to be primarily doctoral students and postdoctoral fellows. Registration is required (see below) but there is no cost to attend. Enrollment in the lectures is open to all PhD students and post-doctoral fellows. Registration in the practical sessions is limited to 20 participants. A brief Statement of Interest will be used to select the Practicum participants, with preference given to graduate students.
For students not in programs where cells or tissue are being studied, a number of lectures may still be relevant including the following topics:
Principles of Experimental Design and Reproducibility of Data
This session addresses designing controlled studies and ensuring rigor and reproducibility in studies
Introduction to Systematic Sampling and Design-Based Stereology
While focussed upon quantifying cell populations in tissue, the principles of randomized studies, systematic sampling, and considerations of sampling error would have general application to studies of any population including patient populations
17-21 September, 2018
No-Cost for Registration
DZNE, Seminar room B.1.119.120
University of Bonn Medical Center, Germany
Registration is closed.
Instruction is by Prof. Dr. Daniel A. Peterson, Professor and Vice-Chairman of Neuroscience and Director, Center for Stem Cell and Regenerative Medicine, The Chicago Medical School. Prof Dr. Peterson has been a Fulbright Senior Scholar and Visiting Professor at the Institute for Reconstructive Neurobiology at Uniklinikum Bonn.
|Confocal microscopes for the practicum are generously provided by Nikon, Zeiss, and MBF Bioscience.
Program of Instruction (detailed curriculum)
|17 September, 2018
||Principles of Widefield and Confocal Microscopy
|09:00 – 17:00
||Principles of Experimental Design, Microscopy, and Fluorescence
|18 September, 2018
||Principles of Quantitative Histology and Systematic Sampling
|09:00 – 17:00
||Image Acquisition, Systematic Sampling, and Estimation of Cell Parameters
Enrollment in the Practicum portion will be limited to 20 students
Students will be divided into AM and PM groups
|19-20 September, 2018
||Practicum on Microscopy and Image Processing
|AM and PM
||Directed group tutorials at the confocal microscopes
|21 September, 2018
||Practicum on Image Processing
|AM and PM
||Post-Processing of Images for Scientific Analysis and Publication
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Promoted by the German Academic Exchange Service, and the Alexander von Humboldt-Foundation
and funded by the Federal Ministry of Education and Research