Workshop in Microscopy and Quantitative Histology in 2019

University of Bonn and the Bonn International Graduate School (BIGS) PhD Program

The goal of this workshop is to combine formalized lectures on the principles of microscopy and quantitative histology (stereology) with practical sessions on the use of confocal microscopes and post-acquisition analysis.  Upon completing this short course, participants should be able to efficiently operate their research instruments, obtain optimal image resolution and quality, and understand the design of a quantitative stereological study.

The participants are expected to be primarily doctoral students and postdoctoral fellows. Registration is required (see below) but there is no cost to attend.  Enrollment in the lectures is open to all PhD students and post-doctoral fellows. Registration in the practical sessions is limited to 20 participants. A brief Statement of Interest will be used to select the Practicum participants, with preference given to graduate students.

For students not in programs where cells or tissue are being studied, a number of lectures may still be relevant including the following topics:

Principles of Experimental Design and Reproducibility of Data

This session addresses designing controlled studies and ensuring rigor and reproducibility in studies

Introduction to Systematic Sampling and Design-Based Stereology

While focussed upon quantifying cell populations in tissue, the principles of randomized studies, systematic sampling, and considerations of sampling error would have general application to studies of any population including patient populations

2-6 September, 2019

No-Cost for Registration

DZNE (hands-on)
University of Bonn Medical Center, Germany

Registration has closed on August 13th, 2019.


Instruction is by Prof. Dr. Daniel A. Peterson, Professor and Vice-Chairman of Neuroscience and Director, Center for Stem Cell and Regenerative Medicine, The Chicago Medical School.  Prof Dr. Peterson has been a Fulbright Senior Scholar and Visiting Professor at the Institute for Reconstructive Neurobiology at Uniklinikum Bonn. He has been appointed an International Ambassador for the University of Bonn.
Confocal microscopes for the practicum are generously provided by Nikon, Zeiss, and MBF Bioscience. nikon_logo-svg mbf_logo_sm_square_400x400

Program of Instruction (detailed curriculum)

2 September, 2019 Principles of Widefield and Confocal Microscopy
09:00 – 17:00 Principles of Experimental Design, Microscopy, and Fluorescence
3 September, 2019 Principles of Quantitative Histology and Systematic Sampling
09:00 – 17:00 Image Acquisition, Systematic Sampling, and Estimation of Cell Parameters

Enrollment in the Practicum portion will be limited to 20 students
Students will be divided into AM and PM groups

4-5 September, 2019 Practicum on Microscopy and Image Processing
AM and PM Directed group tutorials at the confocal microscopes
6 September, 2019 Practicum on Image Processing
AM and PM Post-Processing of Images for Scientific Analysis and Publication


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Promoted by the German Academic Exchange Service, and the Alexander von Humboldt-Foundation

and funded by the Federal Ministry of Education and Research